What Is The Best Way To Avoid Reflux When Drawing Blood
Venipuncture
Venipuncture means the puncturing of a vein for the removal of a venous blood sample. In the medical office, a venipuncture is performed when a large blood specimen is needed for testing. Venipuncture can exist performed by the post-obit two methods:
The vacuum tube method is the fastest and most user-friendly of the three methods and is used most often. This method relies on the use of an evacuated tube, which is a airtight glass or plastic tube that contains a vacuum. The butterfly method is used for difficult draws, such as when a vein is small or sclerosed (hardened). This chapter presents the theory and procedure for both methods.
General Guidelines for Venipuncture
General guidelines that are common to both methods of venipuncture include whatsoever advance preparation, reviewing specimen drove and handling requirements, identification of the patient, reassuring the patient, assembling equipment and supplies, positioning the patient, applying the tourniquet, selecting a site for the venipuncture, obtaining the type of claret specimen required, and following the Occupational Rubber and Health Administration (OSHA) Bloodborne Pathogens Standard.
Get together the Equipment and Supplies
Use merely the appropriate blood tubes as specified by the laboratory directory or manufacturer's instructions accompanying a test system. Substituting blood tubes may non yield the proper blazon of specimen required or may affect the test results, equally shown past the following examples. If serum is required and a tube containing an anticoagulant is used (instead of a tube without an anticoagulant), the claret separates into plasma and cells, rather than serum and cells, and the wrong type of claret specimen is obtained, which necessitates obtaining another specimen from the patient.
The medical assistant should check each blood tube earlier use to ensure that it is not broken, chipped, cracked, or otherwise damaged. Damaged blood tubes are unsuitable for specimen collection and should be discarded. Blood tubes take an expiration appointment (Figure 31-3). The medical assistant should brand certain to cheque the expiration date on the tube to avoid using an outdated blood tube.
The medical banana must exist sure to label each blood tube. An unlabeled specimen is a cause for rejection of the specimen by an exterior laboratory. Two unique identifiers should be used to characterization the specimen. A unique identifier is information that clearly identifies a specific patient, such as the patient's name and appointment of nativity. A specimen tin be labeled by attaching a computerized bar lawmaking label to the specimen (Effigy 31-4, A). The bar lawmaking label includes (at least) ii unique patient identifiers. A specimen tin can also be labeled past handwriting the information on the label, which should include the patient'southward name and appointment of birth (two unique identifiers), the appointment and time of collection, the medical banana's initials, and whatever other information required by the laboratory (Effigy 31-4, B). The information should be printed legibly, and the medical assistant should be sure that the information is accurate to avoid a mix-up of specimens. The medical banana must too complete a laboratory request form to accompany the blood specimen. (NOTE: The medical assistant should follow the medical office policy as to when the tubes should exist labeled. Some offices prefer the tubes exist labeled earlier the specimen is drawn; other offices desire the tubes to be labeled right later on the specimen is obtained.)
Awarding of the Tourniquet
An important footstep in the venipuncture process is the application of the tourniquet. The tourniquet makes the patient'due south veins stand out so that they are easier to palpate. The tourniquet acts equally a "dam," which causes the venous blood to slow downward and pool in the veins in front of the tourniquet. This pooling of blood makes the veins more prominent so that they are more visible and can be palpated.
When applying a tourniquet, it is important to obtain the correct tourniquet tension. The tourniquet should be applied with plenty tension to irksome the venous menstruum without affecting the arterial flow. A tourniquet that is also tight obstructs both venous blood flow and arterial flow, which may result in a specimen that produces inaccurate examination results. A tourniquet that is too loose fails to crusade the veins to stand out enough to be palpated. A correctly applied tourniquet should fit snugly and non pinch the patient's skin.
Guidelines for Applying the Tourniquet
The following guidelines help to ensure successful awarding of the tourniquet:
i. Exercise not apply the tourniquet over sores or burned skin.
two. Place the tourniquet 3 to 4 inches in a higher place the bend in the elbow. This allows adequate room for cleansing the site and performing the venipuncture without the tourniquet getting in the way.
3. Apply the tourniquet so that it is snug, but not so tight that information technology pinches the patient's skin or is otherwise painful to the patient.
4. When applying the tourniquet, enquire the patient to clench his or her fist. This pushes blood from the lower arm into the veins and makes them easier to palpate. You can enquire the patient to clench and unclench the fist a few times; however, vigorous pumping should exist avoided because information technology could lead to hemoconcentration, which could produce inaccurate test results.
5. Never get out the tourniquet on for longer than 1 infinitesimal because this would be uncomfortable for the patient. In addition, prolonged application of the tourniquet causes the venous claret to stagnate, or puddle in one place likewise long—a condition known as venous stasis. When venous stasis occurs, the plasma portion of the blood filters into the tissues, causing hemoconcentration. Hemoconcentration is an increase in the concentration of nonfilterable blood components in the claret vessels, such every bit cherry-red blood cells, enzymes, iron, and calcium, as a result of a decrease in the fluid content of the blood. This can result in inaccurate results for a diverseness of laboratory tests.
6. Ideally, you should remove the tourniquet as before long as a good blood flow is established; notwithstanding, this may not be practical when you are beginning learning the venipuncture procedure. Removing the tourniquet may cause the needle to move such that no more claret can be obtained, and the claret has to exist redrawn. When you are learning the venipuncture procedure, it is improve to await until simply earlier the needle is removed to remove the tourniquet.
7.Always remove the tourniquet before removing the needle from the patient's arm. If the needle is removed first, the force per unit area of the tourniquet causes blood to be forced out of the puncture site and into the surrounding tissue, resulting in a hematoma. A hematoma is defined as a swelling or mass of coagulated blood caused by a interruption in a blood vessel.
viii. After utilize, wipe a tourniquet thoroughly with a disinfectant such as alcohol. Disposable tourniquets are bachelor that are thrown away after 1 utilise.
Site Option for Venipuncture
For most patients, the all-time site to utilize is the veins in the antecubital infinite (Figure 31-viii). If the patient has large, visible antecubital veins, drawing blood is easy. If the patient has pocket-size veins or veins that cannot be palpated, obtaining a claret specimen tin be quite a challenge, fifty-fifty for the most experienced medical assistant.
The antecubital space is the surface of the arm in front of the elbow. The antecubital veins by and large have a wide lumen and are shut to the surface of the pare, which makes them easily accessible. In addition, these veins typically accept thick walls, making them less probable to collapse. Using the antecubital space spares the patient unnecessary pain because the skin is less sensitive there than at other sites, such as the back of the hand. The medical banana should not be misled past the presence in some patients of many modest, very blue "spidery" veins that lie close to the surface of the skin. These veins are non suitable for performing a venipuncture. The antecubital veins lie beneath these veins.
The best vein to utilise in the antecubital space is the median cubital. The median cubital is a prominent vein in the middle of the antecubital space and does not roll (see Figure 31-eight). At times, however, the median cubital vein cannot be used, for example, when it lies deep in the tissues and cannot be palpated or is scarred from repeated venipunctures.
The cephalic and basilic veins are located on opposite sides of the antecubital space and provide an alternative site when the median cubital vein is unavailable. The cephalic vein is located on the thumb side of the antecubital space, and the basilic vein is located on the piddling finger side of the antecubital infinite. The disadvantage of these "side" veins is that they tend to curl or movement abroad from the needle, escaping puncture. To forestall rolling, house pressure should be applied below and to the side of the vein to stabilize information technology as the needle is inserted.
The brachial artery likewise is located in the antecubital space, simply it lies deeper in the tissues. This is the avenue that is used to measure blood pressure. Earlier performing a venipuncture, palpate for the presence of this artery. In contrast to a vein, an artery pulsates, is more elastic, and has a thicker wall than a vein. If the brachial artery is inadvertently punctured, the patient feels more than the usual amount of pain, and the claret is bright blood-red and comes out in pulsing movements. If this situation occurs, the tourniquet should be removed and then the needle. Force per unit area with a gauze pad should be applied for four to 5 minutes.
Guidelines for Site Selection
Specific guidelines should exist followed to facilitate the option of a skillful vein:
1. Ensure that the lighting is acceptable. Good lighting facilitates inspection of the veins.
2. Ensure that the veins "stand out" as much every bit possible. Earlier locating a venipuncture site, always apply the tourniquet, and take the patient make a fist. This combination makes the veins more than prominent.
three. Examine the antecubital veins of both artillery. The best site to perform a venipuncture varies with each individual. The patient may have larger veins in 1 arm than in the other. It is advisable to ask the patient whether he or she has had a venipuncture before. Nearly adults have had previous venipunctures and know which of their veins are best to apply and which should be avoided. Listen to and evaluate information offered by the patient.
iv. Use inspection and particularly palpation to select a vein. A vein does not accept to be seen to be a proficient pick. If you cannot see a vein, palpation alone can be used to locate information technology. A vein feels similar an elastic tube that "gives" nether the pressure of the fingertips.
5. Ever palpate for the median cubital vein (middle vein) first. It commonly is bigger, is anchored better, bruises less, and poses the smallest run a risk of injuring underlying structures (due east.g., nerves and arteries) than the other veins. Because of this, if the patient's median cubital vein cannot exist seen but still can be palpated, it should exist used as the offset option when selecting a vein. If the median cubital vein is skillful in both arms, select the i that appears the fullest. The cephalic vein located on the pollex side is the next best vein choice because information technology does non gyre and trample as hands as the basilic vein. The basilic vein, located on the piddling finger side of the antecubital space, is the to the lowest degree desirable venipuncture site in the antecubital space. Branches of the median nervus may lie close to this vein in some individuals. In addition, the basilic vein lies in shut proximity to the brachial avenue. Both of these conditions pose a adventure of injury to underlying structures when blood is drawn from the basilic vein.
6. Thoroughly appraise the patient'southward veins. To assess a vein every bit a possible site for venipuncture, place one or 2 fingertips (index and middle fingers) over it and printing lightly, and so release pressure level. Do not employ your pollex to palpate the vein because it is not as sensitive as the index finger. To exist suitable for a venipuncture, the vein should experience round, firm, elastic, and engorged. When you depress and release an engorged vein, it should spring back in a rounded, filled state.
7. Make up one's mind the size, depth, and direction of the vein. When a suitable vein has been located, it should exist palpated thoroughly and advisedly to determine the direction of the vein and to judge the size and depth of the vein. Palpate and trace the path of the vein several times by rolling your index finger back and forth over the vein to determine its size. Audit and palpate the vein for problems. Some veins that appear suitable at kickoff sight feel pocket-size, hard, bumpy, or flat when palpated.
8. Map the location of the site. After locating an acceptable vein, mentally "map" the location of the puncture site on the patient's arm with "skin marks." This technique is particularly helpful if the vein cannot be seen, simply only palpated. The puncture site may exist located on or next to a peel marking, such as a freckle, a small wrinkle, or a pigmented expanse.
nine. Do not leave the tourniquet on for longer than 1 minute. When first learning the venipuncture procedure, y'all may demand to perform numerous assessments of the patient'due south arms to locate the best vein. After each assessment, remove the tourniquet for approximately 2 minutes to allow normal circulation of the blood to occur. This prevents patient discomfort and hemoconcentration, which can pb to inaccurate results for a variety of laboratory tests.
ten. If a practiced vein cannot be constitute, the following techniques can be employed to make the veins more prominent:
Alternative Venipuncture Sites
If it is incommunicable to locate a suitable vein in the antecubital infinite, alternative sites are available, including the inner forearm, the wrist area above the thumb, and the dorsum of the paw (Figure 31-nine). These alternative veins are smaller and accept thinner walls than the antecubital veins and should be used for venipuncture but when all possibilities for obtaining the claret specimen at the antecubital site have been considered. If the medical assistant is able to palpate a small vein in the antecubital space, information technology may exist possible to obtain blood there using the butterfly method of venipuncture.
The hand veins, in particular, should exist used simply as a concluding resort. The veins of the paw have a trend to gyre because they are non supported by much tissue and are close to the surface of the skin. This makes them more than difficult to stick. In addition, an abundant supply of nerves is nowadays in the hands, which makes this procedure more uncomfortable for the patient. Mitt veins tend to have thin walls, which makes them more susceptible to collapsing, bruising, and phlebitis. In some patients, however, particularly the obese and the elderly, the mitt veins may be the only attainable site.
OSHA Prophylactic Precautions
The OSHA Bloodborne Pathogens Standard presented in Affiliate 17 must exist carefully followed during the venipuncture process to avoid exposure to bloodborne pathogens. The following OSHA requirements employ specifically to the venipuncture procedure and to separation of serum from whole claret (see after):
ane. Wear gloves when it is reasonably anticipated that yous will have paw contact with blood.
2. Avoid hand-to-mouth contact, such as eating, drinking, or applying makeup, while working with blood specimens.
3. Wearable a face up shield or mask in combination with an middle protection device whenever splashes, spray, splatter, or droplets of claret may be generated.
four. Perform all procedures involving blood in a manner so as to minimize splashing, spraying, splattering, and generating aerosol of blood.
5. Bandage cuts and other lesions on the easily before gloving.
half-dozen. Sanitize hands equally soon as possible after removing gloves.
7. If your hands or other skin surfaces come in contact with blood, wash the expanse every bit soon as possible with soap and h2o.
8. If your mucous membranes (due east.g., eyes, nose, mouth) come in contact with blood, flush them with water equally soon as possible.
nine. Do non bend, break, or shear contaminated venipuncture needles.
10. Practise not recap a contaminated venipuncture needle.
11. Locate the sharps container as close as possible to the area of use. Immediately after use, place the contaminated venipuncture needle (and plastic holder) in the biohazard sharps container.
12. Place blood specimens in containers that forbid leakage during collection, treatment, processing, storage, transport, and shipping.
thirteen. Handle all laboratory equipment and supplies properly and with intendance as indicated by the manufacturer. For instance, await until the centrifuge comes to a complete stop before opening information technology.
14. Exercise not shop food in refrigerators where testing supplies or specimens are stored.
15. If you are exposed to blood, report the incident immediately to your md-employer.
Vacuum Tube Method of Venipuncture
The vacuum tube method is frequently used to collect venous blood specimens. This method is considered ideal for collecting blood from normal healthy antecubital veins that are adequate in size to withstand the pressure of the vacuum in the evacuated tube. Procedure 31-1 outlines the venipuncture vacuum tube method. The vacuum tube system consists of a collection needle, a plastic holder, and an evacuated tube (Figure 31-11). One commercially available vacuum tube arrangement is the Vacutainer Organization (Becton Dickinson, Franklin Lakes, NJ).
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